Review



hpgk lifeact egfp wpre sequence  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc hpgk lifeact egfp wpre sequence
    Hpgk Lifeact Egfp Wpre Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/pmc12450470-160-31-36
    Average 93 stars, based on 52 article reviews
    hpgk lifeact egfp wpre sequence - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    other:

    Article Title: Differential stiffness between brain vasculature and parenchyma promotes metastatic infiltration through vessel co-option.
    Article Snippet: In brain metastasis, cancer cells remain in close contact with the existing vasculature and can use vessels as migratory paths—a process known as vessel co-option.. However, the mechanisms regulating this form of migration are poorly understood.. Here we use ex vivo brain slices and an organotypic in vitro model for vessel co-option to show that cancer cell invasion along brain vasculature is driven by the difference in stiffness between vessels and the brain parenchyma.

    Article Title: Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes
    Article Snippet: Samples were imaged with a Zeiss 540 Crossbeam at 5 kV acceleration voltage, a 700 pA current, and a secondary electron detector at 0° (top view) and 54° tilt (side view) respectively.

    Transduction:

    Article Title: P300 Modulates Endothelial Mechanotransduction of Fluid Shear Stress
    Article Snippet: Purpose P300 is a lysine acetyltransferase that plays a significant role in regulating transcription and the nuclear acetylome.. While P300 has been shown to be required for the transcription of certain early flow responsive genes, relatively little is known about its role in the endothelial response to hemodynamic fluid stress.. Here we sought to define the role of P300 in mechanotransduction of fluid shear stress in the vascular endothelium.

    Article Title: Single cell migration along and against confined haptotactic gradients
    Article Snippet: .. MCF10A-LifeAct-GFP cells were produced by transduction of MCF10A cells with lentiviral particles generated in HEK293T after transient transfection of pLenti.PGK.LifeAct-GFP.W (Addgene, #51010) using Lipofectamine 3000 (ThermoFisher, #L3000008). .. Afterwards, the stable MCF10A-LifeAct-GFP cell line was obtained from a single cell colony expressing medium GFP fluorescence intensity sorted through flow cytometry (FACS) using BD FACSDiva 8.0.1 software.

    Plasmid Preparation:

    Article Title: P300 Modulates Endothelial Mechanotransduction of Fluid Shear Stress
    Article Snippet: Purpose P300 is a lysine acetyltransferase that plays a significant role in regulating transcription and the nuclear acetylome.. While P300 has been shown to be required for the transcription of certain early flow responsive genes, relatively little is known about its role in the endothelial response to hemodynamic fluid stress.. Here we sought to define the role of P300 in mechanotransduction of fluid shear stress in the vascular endothelium.

    Article Title: Cytoplasmic accumulation and plasma membrane association of anillin and Ect2 promote confined migration and invasion
    Article Snippet: .. The following plasmids were 630 purchased from Addgene: pLenti.PGK.LifeAct-GFP.W (plasmid #51010), pLenti.PGK.H2B-631 mCherry (plasmid # 51007), pQC NLS mCherry IX (plasmid #37354), pEGFP-Anillin (plasmid 632 #68027), tetO-FUW-eGFP-RHOA-Q63L (plasmid #73081), RhoA2G FRET biosensor (plasmid 633 #40176, #40179), psPAX2 (plasmid #12260), and pMD2.G (plasmid #12259), VSV.G (plasmid 634 #14888), pLV-EF1α-IRES-puro (plasmid #85132), pLV-EF1α-IRES-Hygro (plasmid #85134). ..

    Article Title: Mechanical Intercellular Communication via Matrix‐Borne Cell Force Transmission During Vascular Network Formation
    Article Snippet: For inhibition studies, PF‐573228 (10 μ m ), GSK205 (10 μ m ; Medchem Express, Monmouth Junction, NJ), and GsMTx‐4 (5 μ m ; Abcam, Cambridge, UK) were supplemented in EGM‐2 and refreshed every 24 h. .. pLenti.PGK.LifeAct‐GFP.W was a gift from Rusty Lansford (Addgene plasmid #51 010). .. To generate lentivirus, plasmids were co‐transfected with pCMV‐VSVG (a gift from Bob Weinberg, Addgene plasmid #8454), pMDLg/pRRE, and pRSV‐REV (gifts from Didier Trono, Addgene plasmid #12 251 and #12 253 [ , ] ) in 293T cells using the calcium phosphate precipitation method.

    Sequencing:

    Article Title: Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes
    Article Snippet: Samples were imaged with a Zeiss 540 Crossbeam at 5kV acceleration voltage, a 700pA current, and a secondary electron detector at 0degree (top view) and 54degree tilt (side view) respectively. .. The SV40-puro cassette comprising SV40 promoter and puromycin resistance gene sequences in pLenti-INV3700-mCherry ( ) was replaced with the hPGK-LifeAct-EGFP-WPRE sequence from pLenti.PGK.LifeAct-GFP.W (Addgene #51010; ) using In-Fusion HD Cloning Kit (Takara). ..

    Cloning:

    Article Title: Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes
    Article Snippet: Samples were imaged with a Zeiss 540 Crossbeam at 5kV acceleration voltage, a 700pA current, and a secondary electron detector at 0degree (top view) and 54degree tilt (side view) respectively. .. The SV40-puro cassette comprising SV40 promoter and puromycin resistance gene sequences in pLenti-INV3700-mCherry ( ) was replaced with the hPGK-LifeAct-EGFP-WPRE sequence from pLenti.PGK.LifeAct-GFP.W (Addgene #51010; ) using In-Fusion HD Cloning Kit (Takara). ..

    Produced:

    Article Title: Single cell migration along and against confined haptotactic gradients
    Article Snippet: .. MCF10A-LifeAct-GFP cells were produced by transduction of MCF10A cells with lentiviral particles generated in HEK293T after transient transfection of pLenti.PGK.LifeAct-GFP.W (Addgene, #51010) using Lipofectamine 3000 (ThermoFisher, #L3000008). .. Afterwards, the stable MCF10A-LifeAct-GFP cell line was obtained from a single cell colony expressing medium GFP fluorescence intensity sorted through flow cytometry (FACS) using BD FACSDiva 8.0.1 software.

    Generated:

    Article Title: Single cell migration along and against confined haptotactic gradients
    Article Snippet: .. MCF10A-LifeAct-GFP cells were produced by transduction of MCF10A cells with lentiviral particles generated in HEK293T after transient transfection of pLenti.PGK.LifeAct-GFP.W (Addgene, #51010) using Lipofectamine 3000 (ThermoFisher, #L3000008). .. Afterwards, the stable MCF10A-LifeAct-GFP cell line was obtained from a single cell colony expressing medium GFP fluorescence intensity sorted through flow cytometry (FACS) using BD FACSDiva 8.0.1 software.

    Transfection:

    Article Title: Single cell migration along and against confined haptotactic gradients
    Article Snippet: .. MCF10A-LifeAct-GFP cells were produced by transduction of MCF10A cells with lentiviral particles generated in HEK293T after transient transfection of pLenti.PGK.LifeAct-GFP.W (Addgene, #51010) using Lipofectamine 3000 (ThermoFisher, #L3000008). .. Afterwards, the stable MCF10A-LifeAct-GFP cell line was obtained from a single cell colony expressing medium GFP fluorescence intensity sorted through flow cytometry (FACS) using BD FACSDiva 8.0.1 software.



    Similar Products

    93
    Addgene inc hpgk lifeact egfp wpre sequence
    Hpgk Lifeact Egfp Wpre Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/pmc12450470-160-31-36
    Average 93 stars, based on 1 article reviews
    hpgk lifeact egfp wpre sequence - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc plenti pgk lifeact gfp w
    Plenti Pgk Lifeact Gfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/pmc12450470-160-34-36
    Average 93 stars, based on 1 article reviews
    plenti pgk lifeact gfp w - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc lifeact gfp
    Lifeact Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/bio_rxiv__2025__08__11__669624-124-21-23
    Average 93 stars, based on 1 article reviews
    lifeact gfp - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc addgene plenti pgk lifeact gfp w
    Addgene Plenti Pgk Lifeact Gfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/pmc12404997__41563_2025_2269_MOESM1_ESM-7-6-6
    Average 93 stars, based on 1 article reviews
    addgene plenti pgk lifeact gfp w - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc glass pipette
    Glass Pipette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+lifeact+gfp+w/pLenti%2EPGK%2ELifeAct-GFP%2EW+(Plasmid+%2351010)/bio_rxiv__2025__01__07__631500-198-11-4
    Average 93 stars, based on 1 article reviews
    glass pipette - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results